Searching the RRID Resource Information Network
Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.
New England Biolabs 2015. Determining Genome Targeting Efficiency using T7 Endonuclease I (M0302). protocols.io https://dx.doi.org/10.17504/protocols.io.cqpvvmCopy Citation Copied
URL: https://dx.doi.org/DOI:10.17504/protocols.io.cqpvvm
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: T7 Endonuclease I recognizes and cleaves non-perfectly matched DNA. This protocol describes how to determine genome targeting efficiency by digesting annealed PCR products with T7 Endonuclease I. In the first step PCR products are produced from the genomic DNA of cells whose genomes were targeted using Cas9, TALEN, ZFN etc. In the second step, the PCR products are annealed and digested with T7 Endonuclease I. Fragments are analyzed to determine the efficiency of genome targeting.
Affiliations: New England Biolabs
External URL: https://www.neb.com/protocols/2014/08/11/determining-genome-targeting-efficiency-using-t7-endonuclease-i
Version: 1
Publication Date: 2015
Expand AllComing soon.
Check
for resource mentions.
Coming soon.
Source:
The NIDDK Information Network (dkNET) serves the needs of basic and clinical investigators by providing seamless access to large pools of data and research resources relevant to the mission of The National Institute of Diabetes Digestive and Kidney Diseases (NIDDK). dkNET is hosted at University of California San Diego, and is supported by NIH NIDDK grant U24DK097771.
Email: info_at_ dknet.org
