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Protocol Name
DOI:DOI:10.17504/protocols.io.ch2t8d RRID Copied  
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New England  Biolabs 2014. In vitro digestion of DNA with Cas9 Nuclease, S. pyogenes (M0386). protocols.io https://dx.doi.org/10.17504/protocols.io.ch2t8d
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Protocol Information

URL: https://dx.doi.org/DOI:10.17504/protocols.io.ch2t8d

Authors: New England Biolabs

Group: New England Biolabs (NEB)

Summary: Cas9 Nuclease, S. pyogenes (Cas9), is a double-stranded DNA endonuclease that is guided to its target by sequence complementarity of a small RNA loaded into the protein. This protocol describes how to digest double-stranded DNA in vitro using Cas9 and a single guide RNA (sgRNA).

Affiliations: New England Biolabs

External URL: https://www.neb.com/protocols/2014/05/01/in-vitro-digestion-of-dna-with-cas9-nuclease-s-pyogenes-m0386

Version: 1

Publication Date: 2014

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