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Maira Rivera, Javiera Reyes, Paula Blazquez-Sanchez, Cesar A Ramirez-Sarmiento 2021. Recombinant expression and purification of codon-optimized M-MLV and Mashup. protocols.io https://dx.doi.org/10.17504/protocols.io.bsernbd6Copy Citation Copied
URL: https://dx.doi.org/DOI:10.17504/protocols.io.bsernbd6
Authors: Maira Rivera, Javiera Reyes, Paula Blazquez-Sanchez, Cesar A Ramirez-Sarmiento
Group: Reclone.org (The Reagent Collaboration Network)
Summary: This protocol has been optimized for the recombinant expression of codon-optizimed M-MLV and MashUp RT.The goal of this protocol was to eliminate the use of large volumes for dyalisis and potential issues with the protein crashing out of the solution due to the use of concentrators for buffer exchange of this enzyme into storage conditions. M-MLV RT has been further functionally tested and works as expected. The sequence of the plasmid encoding the codon-optimized M-MLV RT enzyme can be found here.
Affiliations: Institute for Biological and Medical Engineering, Pontificia Universidad Católica de Chile, Institute for Biological and Medical Engineering, Pontificia Universidad Católica de Chile, Institute for Biological and Medical Engineering, Pontificia Universidad Católica de Chile, Institute for Biological and Medical Engineering, Pontificia Universidad Católica de Chile
Version: 2
Publication Date: 2021
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