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URL: https://dx.doi.org/DOI:10.17504/protocols.io.brtam6ie

Authors: Anthony Brusa, Eric Patterson

Summary: This protocol was developed for the detection of Amaranthus palmeriseeds in mixed pools with other Amaranthus sp. seeds. This is a bulk testing protocol; each pool of seeds generates one data point, the presence or absence of A. palmeri in that sample. Testing pools should be assembled based on the relevant information (e.g. location, population, seed lot, seed supplier, etc.). Controls are extremely important for KASP based assays; this protocol requires positive, negative, and no-template controls. We strongly recommend running 8 controls for the rare allele being detected (e.g. A. palmeri) to aid in data analysis.The protocols may be extended to any species for which appropriate species-specific KASP markers have been developed. DNA extraction protocols were performed using the MPBio FastDNA Plant Kit, but other methods may be substituted.

Affiliations: University of Minnesota - Twin Cities, Michigan State University

Version: 2

Publication Date: 2021

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