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Roey Angel, Eva Petrova 2021. cDNA synthesis using SuperScript™ IV. protocols.io https://dx.doi.org/10.17504/protocols.io.brqum5wwCopy Citation Copied
URL: https://dx.doi.org/DOI:10.17504/protocols.io.brqum5ww
Authors: Roey Angel, Eva Petrova
Group: SoWa RI Anaerobic and Molecular Microbiology (public)
Summary: The following protocol is intended as a downstream application for our Purification of RNA from a DNA/RNA Extract protocol. This protocol describes how to synthesise a first-strand non-specific complementary DNA (cDNA) from a purified RNA extract using SuperScript IV Reverse Transcriptase. The second strand synthesis is usually not required for most downstream applications. This protocol is a simplified and condensed version of the full protocol provided by the manufacturer.
Affiliations: Soil and Water Research Infrastructure, Soil and Water Research Infrastructure
Version: 2
Publication Date: 2021
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