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Andrew Adey, Casey Thornton 2021. sciMAP-ATAC. protocols.io https://dx.doi.org/10.17504/protocols.io.brhxm37nCopy Citation Copied
URL: https://dx.doi.org/DOI:10.17504/protocols.io.brhxm37n
Authors: Andrew Adey, Casey Thornton
Summary: High-throughput single cell genomic assays resolve the heterogeneity of cell states in complex tissues, however, the spatial orientation within the network of interconnected cells is lost. As cell localization is a necessary dimension in understanding complex tissues and disease states, we present a tool for highly scalable spatially-resolved single cell profiling of chromatin state. We use high density multiregional sampling to perform single-cell combinatorial indexing on Microbiopsies Assigned to Positions for the Assay for Transposase Accessible Chromatin (sciMAP-ATAC) to produce single-cell data of equivalent quality to non-spatial single-cell ATAC-seq.
Affiliations: Oregon Health & Science University, Oregon Health & Science University
Version: 2
Publication Date: 2021
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