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Steven Burgess 2020. SDS-PAGE gel electrophoresis. protocols.io https://dx.doi.org/10.17504/protocols.io.bqiemubeCopy Citation Copied
URL: https://dx.doi.org/DOI:10.17504/protocols.io.bqiemube
Authors: Steven Burgess
Group: Ag SynBio Lab UIUC
Summary: SDS-PAGE gel electrophoresis protocol for analyzing samples from plant leaf tissue via immunofluorescence. In this protocol no Coomassie blue is added to samples, the reason is that this interferes with the fluorescent signal during immunoblot. Instead, samples have already been prepared in Laemmli buffer (minus coomassie, protein extraction procedure), the leading edge of samples can be visualized due to the presence of chlorophyll. Note - When using 15 well, 0.75 mm comb, try to limit the volume loaded to 10 μL to minimize the risk of spillover of protein between wells. - Ensure to wipe the tip on the rim of the sample tube to remove the sample stuck to theoutside of the tip. Literature: http://www.bio-rad.com/webroot/web/pdf/lsr/literature/Bulletin_6040.pdfhttps://www.bio-rad.com/webroot/web/pdf/lsr/literature/10026447.pdf
Affiliations: University of Illinois at Urbana-Champaign
Version: 3
Publication Date: 2020
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