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Lixin Wang, Collin Challis, Honghui Liang, Songlin Li, Charless Fowlkes, Aidan Sullivan, Kumar SR, Yvette Taché 2020. Multicolor adeno-associate virus labeling and 3D digital tracing of enteric plexus in mouse proximal colon. protocols.io https://dx.doi.org/10.17504/protocols.io.bqavmse6Copy Citation Copied
URL: https://dx.doi.org/DOI:10.17504/protocols.io.bqavmse6
Authors: Lixin Wang, Collin Challis, Honghui Liang, Songlin Li, Charless Fowlkes, Aidan Sullivan, Kumar SR, Yvette Taché
Group: SPARC
Summary: Using a multicolor adeno-associate virus system to label the colonic enteric nervous system for digital tracing of individual neurons and nerve fibers in microcircuits in three-dimensions (3D). The methods include viral vectors retro-orbital injection in mice, preparation of colon tissues, microscopy and 3D digital tracing.
Affiliations: University of California, Los Angeles, California Institute of Technology, University of California, Los Angeles, University of California, Los Angeles, University of California, Irvine, MBF, California Institute of Technology, University of California, Los Angeles
Version: 1
Publication Date: 2020
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