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URL: https://dx.doi.org/DOI:10.17504/protocols.io.bq2imyce

Authors: Yu Lin Tan, Oriol Pavón Arocas, Lucille Duquenoy, Tiago Branco

Summary: Here we describe a protocol to perform fluorescence in situ hybridization (FISH) in thin sections from frozen murine brain tissue. The protocol can be divided in several days or can be completed in two successive days: one day for extracting and slicing the brain and a second one for the FISH.

Affiliations: Sainsbury Wellcome Centre for Neural Circuits and Behaviour, University College London, Howland Street, London, W1T 4JG, UK, Sainsbury Wellcome Centre for Neural Circuits and Behaviour, University College London, Howland Street, London, W1T 4JG, UK, Sainsbury Wellcome Centre for Neural Circuits and Behaviour, University College London, Howland Street, London, W1T 4JG, UK, Sainsbury Wellcome Centre for Neural Circuits and Behaviour, University College London, Howland Street, London, W1T 4JG, UK

Version: 1

Publication Date: 2021

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