Resource Summary Report  New Search Previous Search Results

URL: https://dx.doi.org/DOI:10.17504/protocols.io.bpwzmpf6

Authors: Rachel Aronoff, Guy Aidelberg

Group: Coronavirus Method Development Community, XPRIZE Rapid Covid Testing, Guy Aidelberg

Summary: Corona Detective is based upon a molecular amplification strategy inspired by the ‘GMO Detective’ method, in order to detect the virus causing Covid-19. Done not only without complicated equipment, but with a simple +/- fluorescent readout, the Corona Detective is very specific. Furthermore, controls to ensure sensitive detection, without false positives or negatives, are intrinsic to this solution. The final product, strips/plates of tubes with dry reagents, specific for Corona and a control gene (extraction control), can be shipped anywhere, without cold-chain dependence. Critically, monitoring tests could be run by ordinary people, by following this protocol. (Even kids have successfully participated in GMO detective workshops, also soldering their own fluor detectors.)This user guide will allow you to test samples with this system, provided as freeze-dried reaction components in 0.2ml tubes made by this protocol (other formats possible e.g. 8-tube strips, 96-well plates, 1000 reactions, etc.).Human or clinical samples should only be run in settings with access to appropriate biosafety facilities, of course.Acknowledging the JOGL Open Covid 19 Initiative and all the #proj nucleic-acid-amplification team for support.

Affiliations: Hackuarium, CRI (Center for research and interdisciplinarity) Paris

Version: 2

Publication Date: 2020

Expand All Collapse