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Joao Vitor Molino 2020. High GC-content PCR. protocols.io https://dx.doi.org/10.17504/protocols.io.bprimm4eCopy Citation Copied
URL: https://dx.doi.org/DOI:10.17504/protocols.io.bprimm4e
Authors: Joao Vitor Molino
Summary: X7 is a a Pfu-sso7d fusion DNA polymerase that is compatible with uracil-excision cloning and has been used with sucess to amplify our high GC-content sequences and it is a valuable tool for USER clonning.Before startingPlease note that protocols with X7 may differ from protocols with other polymerases. Conditions recommended below were tested with our sequences, mostly high GC-content sequences from Chlamydomonas reinhardtii expression vectors. Please refer to the original work that develop the fusion polymerase. All components should be mixed prior to use. Reference:Nørholm, M. H. H. (2010). A mutant Pfu DNA polymerase designed for advanced uracil-excision DNA engineering. BMC Biotechnology, 10. https://doi.org/10.1186/1472-6750-10-21
Affiliations: University of Zürich
Version: 1
Publication Date: 2020
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