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Trevor Ho, Baojun Wang 2020. Intein-assisted Bisection Mapping (IBM). protocols.io https://dx.doi.org/10.17504/protocols.io.bpqdmms6Copy Citation Copied
URL: https://dx.doi.org/DOI:10.17504/protocols.io.bpqdmms6
Authors: Trevor Ho, Baojun Wang
Summary: Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on four proteins, converting them into AND or NAND logic gates. In this protocol we provide details in executing the IBM method.
Affiliations: University of Edinburgh, University of Edinburgh
Version: 1
Publication Date: 2020
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