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Dima Ter-Ovanesyan, Wendy Trieu , Maia Norman, Roey Lazarovits, George Church, David Walt 2020. Isolation of Extracellular Vesicles from Cell Culture Media by Differential Ultracentrifugation . protocols.io https://dx.doi.org/10.17504/protocols.io.bnr3md8nCopy Citation Copied
URL: https://dx.doi.org/DOI:10.17504/protocols.io.bnr3md8n
Authors: Dima Ter-Ovanesyan, Wendy Trieu , Maia Norman, Roey Lazarovits, George Church, David Walt
Group: Walt Lab
Summary: Extracellular vesicles (EVs) are released by all mammalian cells and are thought to be important mediators ofintercellular communication. There are many methods for isolating EVs from cell culture media, but one of themost commonly used methods continues to be purification based on ultracentrifugation. This approach has the advantage of allowing a large input volume of cell culture media. Here, we provide a detailedprotocol for isolating EVs by differential ultracentrifugation.
Affiliations: Wyss Institute for Biologically Inspired Engineering, Wyss Institute for Biologically Inspired Engineering, Wyss Institute for Biologically Inspired Engineering, Brigham and Women's Hospital, Wyss Institute for Biologically Inspired Engineering, Wyss Institute for Biologically Inspired Engineering, Harvard Medical School Department of Genetics, Wyss Institute for Biologically Inspired Engineering, Brigham and Women's Hospital
Version: 1
Publication Date: 2020
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