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Protocol Name
Commercial automated scRNA-seq workflow
DOI:DOI:10.17504/protocols.io.bnmvmc66 RRID Copied  
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DNA Pipelines R&D, Peter Ellis, Lesley Shirley 2020. Commercial automated scRNA-seq workflow. protocols.io https://dx.doi.org/10.17504/protocols.io.bnmvmc66
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Protocol Information

URL: https://dx.doi.org/DOI:10.17504/protocols.io.bnmvmc66

Authors: DNA Pipelines R&D, Peter Ellis, Lesley Shirley

Summary: This SOP describes the procedure for plate based scRNA-seq performed with a commercial available kit from New England BioLabs. Following library construction, samples are pooled in equivolume and quantified, prior to sequencing on the Illumina HiSeq 4000 platform.

Affiliations: Wellcome Sanger Institute, Wellcome Sanger Institute, Wellcome Sanger Institute

Version: 1

Publication Date: 2020

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