Resource Summary Report  New Search Previous Search Results

URL: https://dx.doi.org/DOI:10.17504/protocols.io.bnb2maqe

Authors: Philip J. Haynes, Kavit H. S. Main, Alice Pyne

Summary: This is part 1 of the "Atomic Force Microscopy of DNA and DNA-Protein Interactions" collection of protocols.Collection Abstract: Atomic force microscopy (AFM) is a microscopy technique that uses a sharp probe to trace a sample surface at nanometre resolution. For biological applications, one of its key advantages is its ability to visualize substructure of single molecules and molecular complexes in an aqueous environment. Here, we describe the application of AFM to determine the secondary and tertiary structure of surface-bound DNA, and it’s interactions with proteins.

Affiliations: London Centre for Nanotechnology, University College London, London WC1H 0AH, UK; Molecular Science Research Hub, Department of Chemistry, Imperial College London, W12 0BZ, UK; Department of Physics and Astronomy, University College London, London, WC1E 6BT, UK, London Centre for Nanotechnology, University College London, London WC1H 0AH, UK; UCL Cancer Institute, University College London, London, WC1E 6DD, UK, Department of Materials Science, Sir Robert Hadfield Building, University of Sheffield, S1 3JD

Version: 1

Publication Date: 2020

Expand All Collapse