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Dane Lyddiard 2020. Thin Layer Chromatography (TLC) Bioautography. protocols.io https://dx.doi.org/10.17504/protocols.io.bmxik7keCopy Citation Copied
URL: https://dx.doi.org/DOI:10.17504/protocols.io.bmxik7ke
Authors: Dane Lyddiard
Summary: A TLC bioautography protocol to test the constituents of plant extracts and essential oils for antibacterial activity was adapted and optimised from the methods described in Smith et al. (2007) and Hamburger & Cordell (1987). The protocol described here has been used successfully with a number of non-fastidious organisms, plant extracts and essential oils. The procedure is economical, easy to undertake and the results easily interpreted. In addition to initially screening plants for antibacterial constituents, the TLC bioautography can inform compound isolation work.References:Hamburger, M. O., Cordell, G. A., 1987. A direct Bioautographic TLC assay for compounds possessing antibacterial activity. Journal of Natural Products 50, 19-22.Smith, J. E., Tucker, D., Watson, K., Jones, G. L., 2007. Identification of antibacterial constituents from the indigenous Australian medicinal plant Eremophila duttonii F. Muell. (Myoporaceae). J Ethnopharmacol 112, 386-393.
Affiliations: University of New England, Australia
Version: 2
Publication Date: 2020
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