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Roey Angel, Eva Petrova 2020. Quantification of 16S rRNA Gene Copies Using ddPCR (EvaGreen-based assay: 338F-805R). protocols.io https://dx.doi.org/10.17504/protocols.io.bmqwk5xeCopy Citation Copied
URL: https://dx.doi.org/DOI:10.17504/protocols.io.bmqwk5xe
Authors: Roey Angel, Eva Petrova
Group: SoWa RI Anaerobic and Molecular Microbiology (public)
Summary: This protocol describes how to quantify 16S rRNA bacterial gene or transcript copy numbers using Droplet Digital PCR technology (ddPCR) from Bio-Rad This is an up-to-date modification of a classical bacterial enumeration qPCR-assay. This assay uses the EvaGreen™ chemistry. The primers are taken from Yu et al. (2005).
Affiliations: Soil and Water Research Infrastructure, Soil and Water Research Infrastructure
Version: 1
Publication Date: 2020
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