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Protocol Name
DOI:DOI:10.17504/protocols.io.bmmxk47n RRID Copied  
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Junfei Xia, Hongrong Yang, Michelle Mu, Nicholas Micovic, Kira E. Poskanzer, James Monaghan, Heather Clark 2021. In vivo imaging of acetylcholine release in the peripheral nervous system with a fluorescent nanosensor. protocols.io https://dx.doi.org/10.17504/protocols.io.bmmxk47n
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Protocol Information

URL: https://dx.doi.org/DOI:10.17504/protocols.io.bmmxk47n

Authors: Junfei Xia, Hongrong Yang, Michelle Mu, Nicholas Micovic, Kira E. Poskanzer, James Monaghan, Heather Clark

Summary: This protocol provides a detailed decription of the experimental procedure, including nanosensor farbication and characterization, in vivo microinjection, and in vivo imaging of acetylcholine release in the submandibular ganglion of mice.

Affiliations: Department of Bioengineering, College of Engineering, Northeastern University, Boston, MA, USA, Department of Bioengineering, College of Engineering, Northeastern University, Boston, MA, USA, Department of Bioengineering, College of Engineering, Northeastern University, Boston, MA, USA, Department of Bioengineering, College of Engineering, Northeastern University, Boston, MA, USA, Department of Biochemistry & Biophysics, University of California, San Francisco, San Francisco, CA, USA; Kavli Insititute for Fundamental Neuroscience, San Francisco, CA, USA, Department of Biology, College of Science, Northeastern University, Boston, MA, USA, Department of Bioengineering, College of Engineering, Northeastern University, Boston, MA, USA; Department of Chemistry and Chemical Biology, College of Science, Northeastern University, Boston, MA, USA.

Version: 1

Publication Date: 2021

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