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URL: https://dx.doi.org/DOI:10.17504/protocols.io.bmghk3t6

Authors: Lora Starrs, Debra Brown, Sarah Popp, Charmaine Simeonovic

Summary: Paraffin sections (4 μm thickness) of formalin-fixed human pancreases were treated with heat/citrate buffer for antigen retrieval. Sdc-1core proteins were detected immunohistochemically using rat anti-mouse CD138 (Sdc-1) mAb (BD Biosciences), with horseradish peroxidase-conjugated rabbit anti-rat Ig (Dako). Background staining was checked using the corresponding isotype control Ig instead of the primary antibody. 3-amino-9-ethylcarbazole (AEC) was used as the chromogen. For morphometry, stained sections were imaged using a light microscope with attached camera (Olympus BX41). Image J software with color deconvolution plugin was used for the quantitative analysis of the % of islet area stained.

Affiliations: The John Curtin School of Medical Research, The Australian National University, The John Curtin School of Medical Research, The Australian National University, The John Curtin School of Medical Research, The Australian National University, The John Curtin School of Medical Research, The Australian National University

External URL: https://doi.org/10.1371/journal.pone.0191360

Version: 3

Publication Date: 2020

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