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Ken Christensen 2020. HisPur Purification of His-Tagged Proteins--CHEM 584. protocols.io https://dx.doi.org/10.17504/protocols.io.bmg7k3znCopy Citation Copied
URL: https://dx.doi.org/DOI:10.17504/protocols.io.bmg7k3zn
Authors: Ken Christensen
Summary: The Thermo Scientific HisPur Ni-NTA Resin enables effective immobilized metal affinity chromatography (IMAC) purification of polyhistidine-tagged proteins from a soluble protein extract. This resin is composed of nickel-charged nitrilotriacetic acid (NTA) chelate immobilized onto 6% crosslinked agarose. The Ni-NTA resin is compatible with native or denaturing conditions and can be used in multiple formats, including conventional gravity-flow chromatography, spin column and FPLC. Ni-NTA resins are commonly chosen for His-tagged-protein purification because of the four metal-binding sites on the chelate, which allow for high-binding capacity and low-metal ion leaching.
Affiliations: Brigham Young University
Version: 1
Publication Date: 2020
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