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URL: https://dx.doi.org/DOI:10.17504/protocols.io.bkcaksse

Authors: Andreea S

Group: iGEM Groningen 2020

Summary: In order to get a relative estimation of the diffusion of our neuropeptide in the soil we can use a soil thin-layer chromatography. In this experiment, developed by Helling & Turner in 1968, a thin-layer chromatography apparatus is utilized to mimic the environment conditions to a certain extent. Using C14 labeled glycines we can use X-ray to determine the distance traveled by the peptide. Use of a small his-tag is unreliable due to the small length of the peptide. As a control we will use two C14 labeled compounds common in the exudate of the plant: Glucose and Glutamic acid. Glucose is considered to diffuse well in the soil whereas glutamic acid has a lower diffusion coefficient (cm/h).Protocol is adapted from Ravanel and colleagues (1999).

Affiliations: University of Groningen

Version: 1

Publication Date: 2020

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