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Andreea S 2020. NLP screening (dot blot). protocols.io https://dx.doi.org/10.17504/protocols.io.bkbfksjnCopy Citation Copied
URL: https://dx.doi.org/DOI:10.17504/protocols.io.bkbfksjn
Authors: Andreea S
Group: iGEM Groningen 2020
Summary: In order to determine if the His-tagged NLP is expressed and secreted by the bacteria, we plan to use the dot blot procedure with anti-His tag anti-bodies. Dot blot is an immunological technique used for detecting proteins directly from the culture supernatant (without gel separation). Thus, the samples are directly spotted on the membrane, making it a high throughput procedure ideal for testing different secretion signals.
Affiliations: University of Groningen
Version: 1
Publication Date: 2020
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