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URL: https://dx.doi.org/DOI:10.17504/protocols.io.bk5qky5w

Authors: Randy True

Group: FloodLAMP, XPRIZE Rapid Covid Testing

Summary: The Inactivation Solution includes TCEP, EDTA, NaOH, and water. It should be made in a chemical fume hood, as the TCEP is caustic. It is very straightforward to make if starting with liquid-form TCEP. We have not made it with powder-form TCEP, which is about 30% cheaper at low volume retail prices from Sigma. We have yet to do long-term stability studies; however, we think it's fine stored at room temperature for at least a few weeks. If yellow Binding Solution does not go clear upon addition to an inactivated sample or PBS+Inactivation Soln, suspect that the Inactivation Solution may have degraded, and repeat with another aliquot or freshly-made Inactivation Solution.It has also been suggested by Albert Yu of Brandeis has recommended using a 1.4N NaOH concentration for saliva instead of 1.15N NaOH. NaOH should be scale up by 1.4/1.15 and water down for this.

Affiliations: FloodLAMP Biotechnologies

Version: 1

Publication Date: 2020

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