Searching the RRID Resource Information Network
Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.
Angel Justiz-Vaillant 2020. Universal Immunoblot analysis for investigating Protein-LAG (SpLAG)-binding to mammalian and avian immunoglobulins.. protocols.io https://dx.doi.org/10.17504/protocols.io.bjsdkna6Copy Citation Copied
URL: https://dx.doi.org/DOI:10.17504/protocols.io.bjsdkna6
Authors: Angel Justiz-Vaillant
Group: University of the West Indies, [email protected]
Summary: A protein that combines the binding capacity of SpA, SpG and SpL is not comercially available. It was created in my laboratory by combining these 3 immunoglobulin-binding proteins to horseradish peroxidase by the periodate method [1]. However, a mixture of SpA, SpG and SpL which are comercially available can have the same effect as universal reagent in immunodetection. 1. Vaillant AJ, McFarlane-Andersonv N, Wisdom B, Mohammed W, Vuma S, et al. (2013) Immunoglobulin-binding Bacterial Proteins (IBP) Conjugates and their Reactivity with Immunoglobulin in Enzyme-Linked Immunosorbent Assays (ELISA). J Anal Bioanal Tech 4: 175. doi:10.4172/2155-9872.1000175
Affiliations: University of the West Indies St. Augustine
Version: 1
Publication Date: 2020
Expand AllComing soon.
Check
for resource mentions.
Coming soon.
Source:
The NIDDK Information Network (dkNET) serves the needs of basic and clinical investigators by providing seamless access to large pools of data and research resources relevant to the mission of The National Institute of Diabetes Digestive and Kidney Diseases (NIDDK). dkNET is hosted at University of California San Diego, and is supported by NIH NIDDK grant U24DK097771.
Email: info_at_ dknet.org
