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URL: https://dx.doi.org/DOI:10.17504/protocols.io.bjk7kkzn

Authors: Angel Justiz-Vaillant

Group: University of the West Indies, [email protected]

Summary: Peroxidase conjugated to proteins L, A and G (SpLAG-HRP) is a new development in the field of biochemistry. This versatile protein binds to more 500 species of animal IgGs. It makes it suitable for the study of zoonosis and the assessment of the antibody production to infectious microorganisms. This immunomarker is not available in a recombinant form as their counterpart SpLA-HRP, SpLG-HRP and SpAG-HRP. It can be used in ELISA, immunohistochemistry, Western blot analysis, Dot blot, and RIA [1,2]. References1. Justiz-Vaillant AA, Akpaka PE, McFarlane-Anderson N, Smikle MF. Comparison of techniques of detecting immunoglobulin-binding protein reactivity to immunoglobulin produced by different avian and mammalian species.West Indian Med J. 2013;62(1):12-20.2. Vaillant AJ, McFarlane-Andersonv N, Wisdom B, Mohammed W, Vuma S, et al. (2013) Immunoglobulin-binding Bacterial Proteins (IBP) Conjugates and their Reactivity with Immunoglobulin in Enzyme-Linked Immunosorbent Assays (ELISA). J Anal Bioanal Tech 4: 175. doi:10.4172/2155-9872.1000175.

Affiliations: University of the West Indies St. Augustine

Version: 1

Publication Date: 2020

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