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URL: https://dx.doi.org/DOI:10.17504/protocols.io.bjjdkki6

Authors: Angel Justiz-Vaillant, Suzette E. Curtello

Group: University of the West Indies, [email protected]

Summary: Salmonella are motile, flagellated rod-shaped Zoonotic pathogens which may survive with or without oxygen and do not absorb crystal violet stain.They are decolourised by alcohol due to their outer lipopolysaccharide membrane and thin peptidoglycan layer.They belong to the family Enterobacteriaceae and is implicated with typhoid fever and food-borne illnesses.This pathogen is associated with intestinal disease which may become fatal and has negatively impact the health of individuals and various economies globally.The poultry industry is most impacted and vulnerable to the onslaught of this pernicious microbe.The Lipopolysaccharide Somatic O antigen, flagellar H and virulent V antigenic structure determines the serotype designate of Salmonella species [1]. A wide range of antisera is used in the location of the O antigen associated with the carbohydrate constituent of Lipopolysaccharide and H protein antigen linked with the flagella of the bacteria.Salmonellosis is an inflammation of the intestinal mucosal lining by Salmonella bacteria infiltration resulting in painful symptoms.Both man and animal are vulnerable.Salmonellosis is caused by ingestion of Salmonella with food (Wall et al., 1994).The main route of transmission of Salmonella microbe is fecal-oral. Ingestion of small concentration of Salmonella such as 6 cells with contaminated food or water will lead to salmonellosis infection [2].References1. Varma JK, Marcus R, Stenzel SA, et al. Highly resistant Salmonella Newport-MDRAmpC transmitted through the domestic US food supply: a FoodNet case-control study of sporadic Salmonella Newport infections, 2002-2003.J Infect Dis. 2006;194(2):222-230. doi:10.1086/505084.2. Chen W, Martinez G, Mulchandani A. Molecular beacons: a real-time polymerase chain reaction assay for detecting Salmonella.Anal Biochem. 2000;280(1):166-172. doi:10.1006/abio.2000.4518

Affiliations: University of the West Indies St. Augustine, University of West Indies. Jamaica

Version: 1

Publication Date: 2020

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