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URL: https://dx.doi.org/DOI:10.17504/protocols.io.bjhkkj4w

Authors: Ying Tang, Carly G.K. Ziegler, Vincent N. Miao, Andrew W. Navia, Joshua D. Bromley, Kenneth J. Wilson, Yilianys Pride, Mohammad Hasan, Taylor Christian, Hannah Laird, Anna Owings, Meredith Sloan, Haley B. Williams, Tanya O. Robinson, George E. Abraham III, Michal Senitko, Sarah C. Glover, Bruce Horwitz, Alex K. Shalek, Jose Ordovas-Montanes

Group: Human Cell Atlas Method Development Community, Coronavirus Method Development Community

Summary: A protocol for recovering viable single cell suspensions from cryopreserved human nasopharyngeal swabs for downstream applications, such as single-cell RNA-seq. The illustrated schematic below details the process.

Affiliations: Boston Children's Hospital, Massachusetts Institute of Technology, Massachusetts Institute of Technology, Massachusetts Institute of Technology, Massachusetts Institute of Technology, University of Mississippi Medical Center, University of Mississippi Medical Center, University of Mississippi Medical Center, University of Mississippi Medical Center, University of Mississippi Medical Center, University of Mississippi Medical Center, University of Mississippi Medical Center, University of Mississippi Medical Center, University of Mississippi Medical Center, University of Mississippi Medical Center, University of Mississippi Medical Center, University of Mississippi Medical Center, Boston Children's Hospital, Massachusetts Institute of Technology, Boston Children's Hospital

Version: 1

Publication Date: 2020

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