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Wiebke Nahrendorf, Philip J Spence 2020. low input ChIP-sequencing of immune cells. protocols.io https://dx.doi.org/10.17504/protocols.io.bja3kignCopy Citation Copied
URL: https://dx.doi.org/DOI:10.17504/protocols.io.bja3kign
Authors: Wiebke Nahrendorf, Philip J Spence
Summary: Cells can stably (and heritably) alter their gene expression profile through epigenetic modifications. Histones package DNA into chromatin and can be post-translationally modified - most prominently by methylation and acetylation. These histone modifications alter chromatin structure and DNA accessibility. We optimised a protocol for reliable high quality chromatin immunoprecipitation followed by DNA sequencing (ChIPseq) starting with just 60,000 monocytes isolated directly from mouse tissues by flow sorting. Our protocol can easily be adapted to other mouse or human cell types to interrogate the genome-wide distribution of histone modifications or transcription factor binding sites in immune cells directly ex vivo.
Affiliations: Institute of Immunology & Infection Research, University of Edinburgh, Edinburgh, United Kingdom, Institute of Immunology & Infection Research, University of Edinburgh, Edinburgh, United Kingdom
Version: 1
Publication Date: 2020
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