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Yves Basset 2020. Suggested field procedures for collecting soil/litter arthropods in a tropical rainforest for long-term monitoring with DNA metabarcoding. protocols.io https://dx.doi.org/10.17504/protocols.io.bj9gkr3wCopy Citation Copied
URL: https://dx.doi.org/DOI:10.17504/protocols.io.bj9gkr3w
Authors: Yves Basset
Summary: Suggested field procedures for collecting soil/litter arthropods in a tropical rainforest for long-term monitoring with DNA metabarcoding.Standardized field protocols to monitor the soil fauna exist but they are not tailored to surveys in tropical rainforests and not designated to obtain samples that can be later processed with metabarcoding. In general, the soil fauna is extracted by Berlese-Tullgren apparatus, while the litter-dwelling and more active fauna is better surveyed with Winkler extractors. Here, we used Berlese-Tullgren which represent the choice method for Collembola but included in our samples both soil and litter.
Affiliations: Smithsonian Tropical Research Institute
Version: 1
Publication Date: 2020
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