Searching the RRID Resource Information Network
Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.
Farnaz Shamsi 2020. Isolation of Stromal Vascular Fraction (SVF) from mouse brown adipose tissue (BAT) for single cell RNA-seq. protocols.io https://dx.doi.org/10.17504/protocols.io.bj64krgwCopy Citation Copied
URL: https://dx.doi.org/DOI:10.17504/protocols.io.bj64krgw
Authors: Farnaz Shamsi
Summary: This protocol outlines the procedure for the isolation of the Stromal Vascular Fraction (SVF) from mouse brown adipose tissue (BAT) for single cell RNA-seq. This protocol uses a combination of Collagenase I and Dispase II to digest freshly isolated BAT. Compared to using Collagenase I alone, this combination results in a more efficient dissociation of the adipose vasculature.
Affiliations: Section on Integrative Physiology and Metabolism, Joslin Diabetes Center, Harvard Medical School, Boston, MA, USA
Version: 1
Publication Date: 2020
Expand AllComing soon.
Check
for resource mentions.
Coming soon.
Source:
The NIDDK Information Network (dkNET) serves the needs of basic and clinical investigators by providing seamless access to large pools of data and research resources relevant to the mission of The National Institute of Diabetes Digestive and Kidney Diseases (NIDDK). dkNET is hosted at University of California San Diego, and is supported by NIH NIDDK grant U24DK097771.
Email: info_at_ dknet.org
