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URL: https://dx.doi.org/DOI:10.17504/protocols.io.bj5dkq26

Authors: Ken Christensen

Summary: General Information: VWR Life Science AMRESCO’s NEXT GEL® products for denaturing gel electrophoresis are proprietary, ready-to-pour solutions comprised of acrylamide, bis-acrylamide, gel buffer, and SDS. The unique chemistry of NEXT GEL® eliminates the need for a stacking gel, thus reducing gel preparation time and extending the separation matrix available for electrophoresis, enabling the resolution of small peptides and high molecular weight proteins in the same gel.NEXT GEL® solutions polymerize upon addition of ammonium persulfate and TEMED and are fully compatible with all standard electrophoresis equipment, SDS-PAGE staining procedures, and downstream applications including 2D electrophoresis, western blot, transfer, protein sequencing, and MALDI analysis. Each NEXT GEL® acrylamide solution is supplied with NEXT GEL® Running Buffer, 20X, which is essential for optimal gel performance.

Affiliations: Brigham Young University

Version: 1

Publication Date: 2020

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