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Protocol Name
Methanol-Chloroform-Water Precipitation
DOI:DOI:10.17504/protocols.io.biknkcve RRID Copied  
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Bryon Drown, Kelleher Research Group 2021. Methanol-Chloroform-Water Precipitation. protocols.io https://dx.doi.org/10.17504/protocols.io.biknkcve
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Protocol Information

URL: https://dx.doi.org/DOI:10.17504/protocols.io.biknkcve

Authors: Bryon Drown, Kelleher Research Group

Group: Kelleher Research Group

Summary: Methanol-chloroform-water (MCW) precipitation is a rapid method for removing interferring species from protein samples prior to LC-MS analysis. MCW precipitation can effectively remove both ionic and non-ionic detergents and salts. Precipitating less than 1 µg protein is challenging yet possible with careful pipetting.

Affiliations: Northwestern University, Northwestern University

Version: 1

Publication Date: 2021

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