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Bryon Drown, Kelleher Research Group 2021. Methanol-Chloroform-Water Precipitation. protocols.io https://dx.doi.org/10.17504/protocols.io.biknkcveCopy Citation Copied
URL: https://dx.doi.org/DOI:10.17504/protocols.io.biknkcve
Authors: Bryon Drown, Kelleher Research Group
Group: Kelleher Research Group
Summary: Methanol-chloroform-water (MCW) precipitation is a rapid method for removing interferring species from protein samples prior to LC-MS analysis. MCW precipitation can effectively remove both ionic and non-ionic detergents and salts. Precipitating less than 1 µg protein is challenging yet possible with careful pipetting.
Affiliations: Northwestern University, Northwestern University
Version: 1
Publication Date: 2021
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