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Xiling Shen, Marcos Negrete, Kun Xiang 2020. Duke - Isolation, Culture, and Maintenance of Patient-Derived Tumor Biopsy. protocols.io https://dx.doi.org/10.17504/protocols.io.bijikckeCopy Citation Copied
URL: https://dx.doi.org/DOI:10.17504/protocols.io.bijikcke
Authors: Xiling Shen, Marcos Negrete, Kun Xiang
Group: NCI PDMC consortium
Summary: This protocol is split into 3 sections: collecting tumor cells, passaging, and cryopreserving organoids. Phase 1: Aim:Collect viable cells from biopsy samples Tumor organoid: Colorectal CancerSource: Human primary tissue Phase 2:Aim: Passage and expand organoid samplesPhase 3:Aim: Organoid cryopreservationTumor organoid: Colorectal CancerSource: Human primary tissue
Affiliations: Duke University, Department of Biomedical Engineering, Duke University, Department of Biomedical Engineering, Duke University, Department of Biomedical Engineering
Version: 2
Publication Date: 2020
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