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Judy Northill, Ian Mackay 2020. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) real-time RT-PCR ORF1ab 2020. protocols.io https://dx.doi.org/10.17504/protocols.io.bgtmjwk6Copy Citation Copied
URL: https://dx.doi.org/DOI:10.17504/protocols.io.bgtmjwk6
Authors: Judy Northill, Ian Mackay
Group: Public Health Virology, Forensic and Scientific Services, Coronavirus Method Development Community
Summary: A real-time RT-PCR to specifically detect SARS-CoV-2 betacoronavirus also called nCoV-2019 or Wuhan seafood market pneumonia virus. Based on sequence MN908947 made available by Professor Yong-Zhen Zhang, Fudan University, Shanghai, China. The target region is within the ORF1ab sequence.NotesAssay is fully optimised (as of 24Jan2020).This test has identified a clinical positive cases of coronavirus disease (COVID-19)
Affiliations: Public Health Virology, Forensic and Scientific Services, Public Health Virology, Forensic and Scientific Services
Version: 1
Publication Date: 2020
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