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Mustapha Bittaye, Sarah Gilbert, Katie Ewer, Teresa Lambe 2020. MERS-CoV Spike Glycoprotein (GP) – ELISA Jenner Clinical SOP Template. protocols.io https://dx.doi.org/10.17504/protocols.io.bgr6jv9eCopy Citation Copied
URL: https://dx.doi.org/DOI:10.17504/protocols.io.bgr6jv9e
Authors: Mustapha Bittaye, Sarah Gilbert, Katie Ewer, Teresa Lambe
Group: Coronavirus Method Development Community
Summary: This Standard Operating Procedure (SOP) describes the techniques used to measure the titres of total human immunoglobulin G (IgG) responses against a full length recombinant clamped MERS-CoV spike glycoprotein (GP) in human plasma and serum samples. The measured antibody titres will serve to indicate the humoral immune response to a candidate ChAdOx1 MERS vaccine encoding the full length spike GP of the MERS-CoV in human subjects. This assay must be performed in accordance with the principles of the International Conference on Harmonisation Tripartite Guideline on Good Clinical Practice and the Human Tissue Act. Informed consent must be in place for samples to be tested in this assay.
Affiliations: The Jenner Institute, University of Oxford, The Jenner Institute, University of Oxford, The Jenner Institute, University of Oxford, The Jenner Institute, University of Oxford
Version: 1
Publication Date: 2020
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