Searching the RRID Resource Information Network
Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.
Cellular Generation and Phenotyping 2020. Culture of established induced pluripotent stem cell lines. protocols.io https://dx.doi.org/10.17504/protocols.io.bgbwjspeCopy Citation Copied
URL: https://dx.doi.org/DOI:10.17504/protocols.io.bgbwjspe
Authors: Cellular Generation and Phenotyping
Group: Cellular Generation and Phenotyping
Summary: This protocol outlines the method for thawing, passaging and cryopreserving established feeder-free induced pluripotent stem cell lines. In this culture method, cells are kept as aggregates throughout the process. Cells are cultured on vitronectin matrix (full length xeno-free or truncated protein) in E8 or TeSR-E8 media. Cells can be cultured with or without Penicillin-Streptomycin in the media. Cell lines cultured using these protocols are suitable to use for downstream applications including differentiation and CRISPR screens.
Affiliations: Wellcome Sanger Institute
Version: 1
Publication Date: 2020
Expand AllComing soon.
Check
for resource mentions.
Coming soon.
Source:
The NIDDK Information Network (dkNET) serves the needs of basic and clinical investigators by providing seamless access to large pools of data and research resources relevant to the mission of The National Institute of Diabetes Digestive and Kidney Diseases (NIDDK). dkNET is hosted at University of California San Diego, and is supported by NIH NIDDK grant U24DK097771.
Email: info_at_ dknet.org
