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Zhichao Zhao Shenao Biotechnology Company, wang jiang 2020. A protocol for rapid western-blot: shorten the time to 1-3 hours. protocols.io https://dx.doi.org/10.17504/protocols.io.bfyxjpxnCopy Citation Copied
URL: https://dx.doi.org/DOI:10.17504/protocols.io.bfyxjpxn
Authors: Zhichao Zhao Shenao Biotechnology Company, wang jiang
Summary: Western blot (WB) is a classical and powerful tool to detect the level of interested protein from among a mixture of proteins. Since its invention in 1979, WB is a time-consuming technique that usually needs 1 to 2 days to obtain the result. Here, being aware of the time spent by the researcher, we share a rapid protocol that shortens the time up to 1 hour for detecting the internal loading control protein actin (42kDa) when using the HRP-conjugated anti-actin antibody. Additionally, the time for detecting other (21kDa-100kDa) could be shortened to 3 hours when using the second HRP-conjugated antibody. In conclusion, this rapid protocol dramatically improved the efficiency of WB without increasing the experiment cost or losing its high data quality.
Affiliations: Shenao Biotechnology Company, University of Chinese Academy of Science
Version: 2
Publication Date: 2020
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