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URL: https://dx.doi.org/DOI:10.17504/protocols.io.bfuwjnxe

Authors: Mohammad Farris Iman Leong Bin Abdullah, Kok Leng Tan, Salbiah Mohd Isa, Nur Sabrina Yusoff, Nelson Jeng Yeou Chear, Darshan Singh

Summary: The protocol describe the steps involved in conducting fasting lipid profile and liver function test from the blood samples of regular kratom users and healthy non-kratom using subjects in this study. This tests were conducted in an ISO 15189-certified laboratory at Advanced Medical and Dental Institute, Universiti Sains Malaysia. The blood parameters which were determined quantitatively included serum triglyceride (TG), total cholesterol (TC), high-density lipoprotein (HDL), total protein (TP), albumin, alkaline phosphatase (ALP), alanine transaminase (ALT), aspartate transaminase (AST), total bilirubin (TB) and direct bilirubin (DB). While globulin was determined from simple subtraction of albumin from total protein value, indirect bilirubin was determined by subtraction of direct bilirubin from total bilirubin value, and low-density lipoprotein (LDL) was calculated via Friedwald equation.

Associated Publications: Abdullah MFILB, Tan KL, Isa SM, Yusoff NS, Chear NJY, Singh D (2020) Lipid profile of regular kratom (Mitragyna speciosa Korth.) users in the community setting. PLoS ONE 15(6): e0234639. doi: 10.1371/journal.pone.0234639

Affiliations: Lifestyle Science Cluster, Advanced Medical and Dental Institute, Universiti Sains Malaysia, Regenerative Medicine Cluster, Advanced Medical and Dental Institute, Universiti Sains Malaysia, Lifestyle Science Cluster, Advanced Medical and Dental Institute, Universiti Sains Malaysia, Centre for Drug Research, Universiti Sains Malaysia, Centre for Drug Research, Universiti Sains Malaysia, Centre for Drug Research, Universiti Sains Malaysia

External URL: https://doi.org/10.1371/journal.pone.0234639

Version: 1

Publication Date: 2020

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