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URL: https://dx.doi.org/DOI:10.17504/protocols.io.bfavjie6

Authors: Joseph Patterson, Allyson Cole-Strauss, John Beck, Caryl Sortwell, Jack Lipton

Group: Coronavirus Method Development Community

Summary: The common methods of sample collection for the detection of SARS-Cov2 are either pharyngeal or nasopharyngeal. Both these methods require specific swabs that are in short supply, and both methods are not easily self-administered. A nasal swab can also be used to collect samples and is relatively easy to self-administer, yet obtaining nasal specific swabs can now also be a challenge. In order to address these issues, our group has optimized a protocol for self-administered nasal collection using buccal swabs that are not currently in high demand. With this method, we have been able to collect human samples with reliable results in RNA extraction based on RNaseP, and can detect SARS-Cov2 in known positive cases of COVID-19.

Affiliations: Michigan State University, Michigan State University, Michigan State University, Michigan State University, Michigan State University

Version: 1

Publication Date: 2020

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