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DOI:DOI:10.17504/protocols.io.bf5qjq5w RRID Copied  
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Priota Islam 2020. Creating Bacterial Glycerol Stocks for Long-term Storage. protocols.io https://dx.doi.org/10.17504/protocols.io.bf5qjq5w
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URL: https://dx.doi.org/DOI:10.17504/protocols.io.bf5qjq5w

Authors: Priota Islam

Group: Behavioural Genomics

Summary: Bacterial glycerol stocks are important for long-term storage. Bacteria on LB agar plate can be stored at 4°C for a few weeks. However, if you want to store bacteria for a longer time, you will need to establish glycerol stocks. The addition of glycerol stabilizes the frozen bacteria, preventing damage to the cell membranes and keeping the cells alive. A glycerol stock of bacteria can be stored stably at -80°C for many years.

Affiliations: Imperial College London

Version: 1

Publication Date: 2020

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