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Frank Ferrer-Gonzalez, Christa Smith 2020. RNA extraction and precipitation for 47 mm PES and polycarbonate filters. protocols.io https://dx.doi.org/10.17504/protocols.io.bervjd66Copy Citation Copied
URL: https://dx.doi.org/DOI:10.17504/protocols.io.bervjd66
Authors: Frank Ferrer-Gonzalez, Christa Smith
Summary: This protocol is a modified version of Bryn Durham et al., 2015 for extracting RNA from filters. This protocol has been tested with 0.22 μm pore size Durapore filters and 2.0 μm polycarbonate filters. Custom synthesized RNA transcript standards are added at the time of extraction and are recovered post-sequencing for quantitative metatranscriptome analysis (Satinsky et al., 2013). To prevent RNA degradation, work in a clean environment with asceptic practices and avoid possible introduction of RNases to samples and reagents.
Affiliations: University of Georgia, University of Georgia
Version: 2
Publication Date: 2020
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