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Max Marrone 2020. Opentrons COVID-19 testing (Randox/qPCR common path, stations A & B, 24 samples). protocols.io https://dx.doi.org/10.17504/protocols.io.bemfjc3nCopy Citation Copied
URL: https://dx.doi.org/DOI:10.17504/protocols.io.bemfjc3n
Authors: Max Marrone
Group: Opentrons COVID-19 Testing
Summary: Opentrons and the Open Medicine Institute are developing an automated high-throughput COVID-19 testing protocol to submit to the FDA for an Emergency Use Authorization as a diagnostic.The standard assay for this type of infectious disease testing is quantitative PCR (qPCR), and in this case reverse transcriptase qPCR since COVID-19 is an RNA virus. After patient samples are collected in public health facilities, doctors offices, and hospitals, they are sent to the lab for processing, which happens in four steps:Sample IntakeRNA ExtractionqPCR SetupRT-qPCR AssayOpentrons OT-2 robots carry out most of this work. However, human operators are needed for some key tasks, like:Moving samples between stationsPreparing certain reagentsRunning the qPCR machineLogging data
Affiliations: Opentrons Labworks
Version: 5
Publication Date: 2020
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