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URL: https://dx.doi.org/DOI:10.17504/protocols.io.be8ujhww

Authors: Joseph Patterson, Allyson Cole-Strauss, John Beck, Caryl Sortwell, Jack Lipton

Group: Coronavirus Method Development Community

Summary: Viral RNA isolation kits used in PCR based tests for the novel coronavirus (SARS-Cov2) are in short supply. Our group sought to identify a method to isolate viral RNA without the use of a kit or other supplies in high demand by clinical labs. Using a TRIzol based RNA extraction, followed by a glycogen precipitation, we are able to isolate a sufficient quality and quantity of RNA for quantitative PCR, and/or droplet digital PCR. This method has been confirmed to work as well as a viral RNA isolation kit, shown to work with SARS-Cov2 RNA-spiked into human samples, and in detecting SARS-Cov2 in known positive cases of COVID-19.

Affiliations: Michigan State University, Michigan State University, Michigan State University, Michigan State University, Michigan State University

Version: 1

Publication Date: 2020

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