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Rachel Howard-Till, Claudia Osorio, Bradley Till 2020. Low-cost tissue collection and genomic DNA extraction for plants. protocols.io https://dx.doi.org/10.17504/protocols.io.bdg9i3z6Copy Citation Copied
URL: https://dx.doi.org/DOI:10.17504/protocols.io.bdg9i3z6
Authors: Rachel Howard-Till, Claudia Osorio, Bradley Till
Summary: This protocol describes low-cost methods for the collection and desiccation of plant tissues and subsequent extraction of genomic DNA. It contains modifications of previously described methods found at https://link.springer.com/book/10.1007/978-3-319-16259-1 and https://link.springer.com/chapter/10.1007/978-3-319-45021-6_14. We have tested this protocol with leaf tissue from flax, Lupinus luteus, Camelina sativa and Berberis darwinii. DNA is suitable for routine molecular biology assays such as PCR and also for next generation sequencing.
Affiliations: Centro de Genómica Nutricional Agroacuícola; https://orcid.org/0000-0003-2568-7980, Instituto de Investigaciones Agropecuarias, Centro de Genómica Nutricional Agroacuícola; https://orcid.org/0000-0002-1300-8285
Version: 1
Publication Date: 2020
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