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Luciano Martelotto 2020. Protocol for nuclei isolation from fresh and frozen tissues for parallel snRNA-Seq and snATAC-Seq on 10x ChromiumTM platform using the same nuclei preparation (UPDATED version). protocols.io https://dx.doi.org/10.17504/protocols.io.bdeni3deCopy Citation Copied
URL: https://dx.doi.org/DOI:10.17504/protocols.io.bdeni3de
Authors: Luciano Martelotto
Group: Human Cell Atlas Method Development Community
Summary: This protocol is an adaptation and extension of the 'Frankenstein' protocol – originally developed for nuclei isolation from fresh and frozen tissue for snRNA-Seq – in order to perform snATAC-Seq on the same nuclei prep. It has been successfully applied to fresh, snap/flash and cryopreserved frozen cell lines as well as to tissue derived from solid tumours and other tissues such as pancreas adenocarcinoma (PDAC), breast cancers, pheochromocytomas/paragangliomas, normal paraganglia, brain organoids, PDAC organoids, ovary, fallopian tube, mouse brain and sperm using the Chromium Platform (10x Genomics).
Affiliations: Harvard Medical School
Version: 4
Publication Date: 2020
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