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Kizhakke Mattada Sathyan, Thomas G. Scott, Michael J. Guertin 2020. Basic Protocol 1: Generation of eGFP-ARF-P2A-TIR1 or ARF-HA-P2A-TIR1 progenitor cells. protocols.io https://dx.doi.org/10.17504/protocols.io.bde4i3gwCopy Citation Copied
URL: https://dx.doi.org/DOI:10.17504/protocols.io.bde4i3gw
Authors: Kizhakke Mattada Sathyan, Thomas G. Scott, Michael J. Guertin
Summary: The first procedure for implementing the ARF-AID system is to establish ARF-TIR1 progenitor cells, as shown in Figure 2A. All the plasmids for integrating ARF-TIR1 into the AAVS1 locus in human cells are available from Addgene. The choice of transfection method varies depending on the cell type; lipofectamine 3000 works efficiently for HEK293T cells.
Affiliations: Biochemistry and Molecular Genetics Department, University of Virginia, Biochemistry and Molecular Genetics Department, University of Virginia, Biochemistry and Molecular Genetics Department, University of Virginia ; Center for Public Health Genomics, University of Virginia; Cancer Center, University of Virginia
Version: 1
Publication Date: 2020
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