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URL: https://dx.doi.org/DOI:10.17504/protocols.io.bcvyiw7w

Authors: Boas Pucker

Group: High molecular weight DNA extraction from all kingdoms

Summary: Plant DNA extraction and preparation for ONT sequencingThis CTAB-based protocol is suitable for extraction of genomic DNA from a wide range of plant species. The DNA quality is sufficient for ONT sequencing after SRE enrichment of long fragments and removal of short fragments. Quality control steps are described as part of this protocol.Successful application for the genome sequencing of the following plant species:Arabidopsis species, Beta vulgaris (sugar beet), Brassica napus (rapeseed/canola), Dioscorea dumetorum (yams), Helichrysum umbraculigerum, mosses, Vitis vinifera (grapevine)References:Siadjeu C.*, Pucker B.*, Viehoever P., Albach D. and Weisshaar B. (2020). High contiguity de novo genome sequence assembly of Trifoliate yam (Dioscorea dumetorum) using long read sequencing. Genes. doi:10.3390/genes11030274.Pucker B, Rückert C, Stracke R, Viehöver P, Kalinowski J, Weisshaar B. Twenty-Five Years of Propagation in Suspension Cell Culture Results in Substantial Alterations of the Arabidopsis Thaliana Genome. Genes. 2019. doi:10.3390/genes10090671.Photo provided by Hanna Schilbert (@HSchilbert).

Affiliations: Department of Plant Sciences, University of Cambridge (UK)

External URL: https://www.cebitec.uni-bielefeld.de/~bpucker

Version: 1

Publication Date: 2020

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