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Integrated Islet Distribution Program 2020. Sentinel Flask Preparation for Cold Shipping Protocol of Human Islets. protocols.io https://dx.doi.org/10.17504/protocols.io.bctziwp6Copy Citation Copied
URL: https://dx.doi.org/DOI:10.17504/protocols.io.bctziwp6
Authors: Integrated Islet Distribution Program
Group: Integrated Islet Distribution Program
Summary: To establish a standardized method for the National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK) sponsored research in the Integrated Islet Distribution Program (IIDP) for preparing a sentinel flask of human islets in order to monitor sterility and islet integrity at the production center. This is a representative sample of the islet preparation that has been distributed to investigators and is held at the distributing center as a quality control sample. The islets are cultured in the same shipping media used for islet shipment, for 18 hours at 4-8°C, simulating the cold shipping conditions followed by 2 days at 37°C culture. The flask of islets are verification in the event of a dispute over sterility or islet quality.
Affiliations: Integrated Islet Distribution Program, City of Hope
External URL: https://dx.doi.org/10.17504/protocols.io.bac5iay6; https://iidp.coh.org/Investigators/Policies-Standard-Operating-Procedures
Version: 1
Publication Date: 2020
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