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Oxford HCA, Nicholas Provine, Michael FitzPatrick, Sophie Irwin 2020. Isolation of cells from the epithelial layer of frozen human intestinal biopsies. protocols.io https://dx.doi.org/10.17504/protocols.io.bcb6isreCopy Citation Copied
URL: https://dx.doi.org/DOI:10.17504/protocols.io.bcb6isre
Authors: Oxford HCA, Nicholas Provine, Michael FitzPatrick, Sophie Irwin
Summary: This protocol is for the isolation of the epithelial cells (and associated immune cells within the epithelial layer) from frozen human intestinal pinch biopsies, for use in single-cell sequencing or flow cytometry experiments. This protocol is adapted from Parikh, Antanaviciute, Fawkner-Corbett et al., (2019) Natuer (doi: 10.1038/s41586-019-0992-y). It has been modified specifically with the aim of increasing cell recovery from biopsies of the small intestine.
Affiliations: Oxford University, Oxford University, Oxford University, Oxford University
Version: 1
Publication Date: 2020
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