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Morgan Colp 2020. Single cell isolation and monoclonal culture establishment of Acanthamoeba castellanii using migration on agar plates. protocols.io https://dx.doi.org/10.17504/protocols.io.bc3eiyjeCopy Citation Copied
URL: https://dx.doi.org/DOI:10.17504/protocols.io.bc3eiyje
Authors: Morgan Colp
Group: Protist Research to Optimize Tools in Genetics (PROT-G)
Summary: Isolation of single Acanthamoeba castellanii cells from agar plates for establishment of monoclonal cultures. This protocol can be useful for isolating clones of a mixed wild-type population or of transformants. After isolation, growth can be re-established in typical Neff liquid medium.
Affiliations: Dalhousie University
Version: 1
Publication Date: 2020
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